And Protocols: Ancient Dna: Methods

Once extracted, the DNA must be prepared for Next-Generation Sequencing (NGS).

Synthetic DNA "adapters" are attached to the ends of the fragments, allowing them to bind to the sequencing platform. Ancient DNA: Methods and Protocols

If the sample has low endogenous DNA (e.g., 99% of the DNA is from soil bacteria), researchers use "baits"—RNA probes that match the target genome—to "fish out" the human or animal DNA of interest. 5. Bioinformatic Authentication The final step is proving the DNA is actually ancient. Once extracted, the DNA must be prepared for