"NINAHD" (often stylized as ) refers to Nanopore Identification of N-Acetylation by Hydroxylamine Deacetylation . It is a universal chemical sensing strategy published in ACS Sensors in March 2024 to identify N-acetyl modifications on various biomacromolecules. Overview of NINAHD
For further technical details, the full study is available through the ACS Sensors journal . NinaHD
: It is considered a universal strategy because it can identify N-acetyl modifications on RNA, DNA, proteins, and glycans . "NINAHD" (often stylized as ) refers to Nanopore
: The method uses hydroxylamine to treat N-acetylation, which produces acetohydroxamic acid (AHA) . This AHA molecule then serves as a "reporter" that is specifically sensed by a modified protein nanopore (PBA-modified MspA). : It is considered a universal strategy because
: Unlike traditional analytical methods like mass spectrometry, NINAHD does not require the translocation or unfolding of large molecules, allowing it to identify modifications even on proteins too large to pass through the pore.